Dr. Xi Chen, University of California, Davis
Abstract: Carbohydrates and glycoconjugates play important roles in biology. Obtaining these structures in pure forms will help to elucidate their functions and explore their potential therapeutic applications. We have developed highly efficient one-pot multienzyme (OPME) systems for synthesizing various carbohydrates and glycoconjugates from suitable glycosyltransferase acceptors and simple monosaccharides as donor precursors. These OPME systems can be used in sequential for large-scale production of a library of human milk oligosaccharides and glycolipids, synthesizing glycan epitopes for biological function studies, and glycan engineering of glycoproteins. Our efforts on enzyme characterization, structure studies, and protein engineering of glycosyltransferases as well as their applications on enzymatic and chemoenzymatic synthesis of glycoproteins, glycolipids, and human milk oligosaccharides will be presented. New findings from our collaborative work on glycan microarray-based platform will also be shared.