Biography

myles

CONTACT INFORMATION

Office Phone: 301 405-1811
Office Address: 2504
 Email: mpoulin@umd.edu
Group Website: www.poulinlabumd.com

Assistant Professor

Education

  • 2003 ­– 2007    B.Sc. Biochemistry & Molecular Biology, University of Northern British Columbia
  • 2007 – 2012    Ph.D. Chemistry, University of Alberta (Thesis advisor, Todd Lowary)
  • 2012 – 2016    Postdoc Biochemistry, Albert Einstein College of Medicine, NSERC Postdoctoral Fellow  (Postdoc advisor, Vern Schramm)

Research interest

Bioorganic Chemistry, Enzymology, Carbohydrate Chemistry, Glycobiology, Molecular Biology, Microbiology, Bacterial Biofilm Biosynthesis, Membrane Transport of Polysaccharides, Kinetic Isotope Effects

Major Recognitions and Honors

  •  2016, Dennis Shields Postdoctoral Research Prize, Albert Einstein College of Medicine
  • 2014 – 2016 ,   NSERC Postdoctoral Fellow, Albert Einstein College of Medicine
  • 2010 – 2012,    NSERC Alexander Graham Bell Canada Graduate Scholarship, University of Alberta
  • 2010 – 2012,    President’s Doctoral Prize of Distinction, University of Alberta
  • 2008 – 2011,    Alberta Innovates Technology Futures Graduate Scholarship, University of Alberta
  • 2007 – 2008,    NSERC Postgraduate Scholarship, University of Alberta
  • 2005,  NSERC Undergraduate Student Research Award, University of Northern British Columbia

Research

Chemical biology of Microbial Biofilm Exopoylsaccharides

My laboratory is interested in developing tools to study the biosynthesis of bacterial exopolysaccharides and the role of these exopolysaccharides in biofilm formation and bacterial infections. Projects in the lab use a multidisplinary approach combining synthetic carbohydrate chemistry, enzymology, membrane protein biophysics and molecular biology to study the structure, biosynthesis, membrane transport, and functions of exopolysaccharide that make up the extracellular matrix of bacterial biofilm communities.

 myles_research

A second area of study in my lab focuses on the activity of bifunctional glycosyltransferase (GT) enzymes. Unlike protein and nucleic acid biosynthesis, which use a template strand to control polymer sequence and length, the biosynthesis of polysaccharides is template independent. As a result, polysaccharide biosynthesis often requires multiple GT enzymes, each specific for adding a single monosaccharide to the growing polymer. However, there are a number of polysaccharides that contain a disaccharide repeat unit that are assembled by single bifunctional GT enzymes. Projects in the lab aim to understand how bifunctional GT are able to control polymer sequence and chain length during polysaccharide biosynthesis.

My lab also seeks to develop chemical probes that disrupt exopolysaccharide biosynthesis in order to study their role in biofilm assembly in vivo. We combine mechanism based inhibitor design and transition state analog design approaches to develop inhibitors of the glycosyltransferase enzymes involved in exopolysaccharide biosynthesis.

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